Syphilis-causing strains belong to separate SS14-like or Nichols-like groups as defined by multilocus analysis of 19 Treponema pallidum strains

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Publikace nespadá pod Pedagogickou fakultu, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.
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NECHVÁTAL Lukáš PĚTROŠOVÁ Helena GRILLOVÁ Linda POSPÍŠILOVÁ Petra MIKALOVÁ Lenka STRNADEL Radim KUKLOVÁ Ivana KOJANOVÁ Martina KREIDLOVÁ Miluše VAŇOUSOVÁ Daniela PROCHÁZKA Přemysl ZÁKOUCKÁ Hana KRCHŇÁKOVÁ Alena ŠMAJS David

Rok publikování 2014
Druh Článek v odborném periodiku
Časopis / Zdroj International Journal of Medical Microbiology
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.1016/j.ijmm.2014.04.007
Obor Mikrobiologie, virologie
Klíčová slova Treponema pallidum; Syphilis; Yaws; Molecular typing; Multilocus analysis; SS14-like strains
Popis Treponema pallidum strains are closely related at the genome level but cause distinct diseases. Subspecies pallidum (TPA) is the causative agent of syphilis, subspecies pertenue (TPE) causes yaws while subspecies endemicum (TEN) causes bejel (endemic syphilis). Compared to the majority of treponemal genomic regions, several chromosomal loci were found to be more diverse. To assess genetic variability in diverse genomic positions, we have selected (based on published genomic data) and sequenced five variable loci, TP0304, TP0346, TPO488, TP0515 and TP0558, in 19 reference Treponema pallidum strains including all T. pallidum subspecies (TPA, TPE and TEN). Results of this multilocus analysis divided syphilitic isolates into two groups: SS14-like and Nichols-like. The SS14-like group is comprised of SS14, Grady, Mexico A and Philadelphia 1 strains. The Nichols-like group consisted of strains Nichols, Bal 73-1, DAL-1, MN-3, Philadelphia 2, Haiti B and Madras. The TP0558 locus was selected for further studies because it clearly distinguished between the SS14- and Nichols-like groups and because the phylogenetic tree derived from the TP0558 locus showed the same clustering pattern as the tree constructed from whole genome sequences. In addition, TP0558 was shown as the only tested locus that evolved under negative selection within TPA strains. Sequencing of a short fragment (573 bp) of the TP0558 locus in a set of 25 clinical isolates from 22 patients collected in the Czech Republic during 2012-2013 revealed that clinical isolates follow the SS14- and Nichols-like distribution. (C) 2014 Elsevier GmbH. All rights reserved.
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