Realistic Simulation of Polymicrobial Wound Infections: A 3D-Bioprinted Approach
| Autoři | |
|---|---|
| Rok publikování | 2025 |
| Druh | Konferenční abstrakty |
| Citace | |
| Přiložené soubory | |
| Popis | The prevalence of non-healing wounds represents a significant and escalating challenge within contemporary healthcare. A primary factor contributing to delayed wound closure is the infection of the wound and subsequent formation of the microbial biofilms. To advance successful and personalized therapeutic strategies for chronic wounds, the development of an appropriate biofilm model that realistically simulates wound infection is necessity. Our solution lies in 3D bioprinting technology, which offers an unparalleled opportunity to construct complex, spatial structures incorporating mixed bacterial populations, extracellular polysaccharides, and other crucial components found within wound environments. We successfully developed a 3D-bioprinted model of polymicrobial biofilms specifically engineered to mimic the characteristics of chronic wound infections. This innovative model utilizes a readily achievable modification of a standard 3D printer (in our case Prusa MK4S) for the precise deposition of bio-inks. Verification of its utility was conducted using a set of microbial species commonly isolated from chronic wound infections to mimic both the mono-microbial and poly-microbial infections: S. aureus, Str. dysgalactiae, E. faecalis, Sten. maltophilia, Kl. pneumoniae, M. morganii, and E. coli, employing alginate and poloxamer matrices. Bacterial quantification was performed through conventional plate counting on both non-selective and species-specific selective media. For robust reproducibility, we standardized our matrix compositions to 1% alginate (hardened with 1% CaCl2) and 30% poloxamer, both prepared in Mueller-Hinton Broth. Both matrices demonstrated suitability for all tested species, yielding bacterial concentrations five to six orders of magnitude greater than the initial inoculum. An optimal bacterial inoculum of 1.5×10^3 CFU enabled the quantification of both mono- and poly-microbial biofilms. While most species exhibited marginally superior growth in the alginate matrix, E. faecalis consistently favored the poloxamer matrix. |
| Související projekty: |