Freeze-drying as suitable method to achieve ready-to-use yeast biosensors for androgenic and estrogenic compounds

Logo poskytovatele
Logo poskytovatele

Varování

Publikace nespadá pod Pedagogickou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
Autoři

JARQUE ORTIZ Sergio BITTNER Michal HILSCHEROVÁ Klára

Rok publikování 2016
Druh Článek v odborném periodiku
Časopis / Zdroj Chemosphere
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www http://www.sciencedirect.com/science/article/pii/S0045653516300376
Doi http://dx.doi.org/10.1016/j.chemosphere.2016.01.038
Obor Vliv životního prostředí na zdraví
Klíčová slova Recombinant yeast assay; Freeze-drying; Estrogen; Androgen; Long-term; Ready-to-use
Popis Recombinant yeast assays (RYAS) have been proved to be a suitable tool for the fast screening of compounds with endocrine disrupting activities. However, ready-to-use versions more accessible to less equipped laboratories and field studies are scarce and far from optimal throughputs. Here, we have applied freeze-drying technology to optimize RYA for the fast assessment of environmental compounds with estrogenic and androgenic potencies. The effects of different cryoprotectants, initial optical density and long-term storage were evaluated. The study included detailed characterization of sensitivity, robustness and reproducibility of the new ready-to-use versions, as well as comparison with the standard assays. Freeze-dried RYAS showed similar dose-responses curves to their homolog standard assays, with Lowest Observed Effect Concentration (LOEC) and Median effective Concentration (EC50) of 1 nM and 7.5 nM for testosterone, and 0.05 nM and 0.5 nM for 17 beta-estradiol, respectively. Freeze-dried cells stored at 4 degrees C retained maximum sensitivity up to 2 months, while cells stored at -18 degrees C showed no decrease in sensitivity throughout the study (10 months). This ready-to-use RYA is easily accessible and may be potentially used for on-site applications.
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.