Highly Sensitive Laser Scanning of Photon-Upconverting Nanoparticles on a Macroscopic Scale

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Publikace nespadá pod Pedagogickou fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
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SEDLMEIER Andreas HLAVÁČEK Antonín BIRNER Lucia MICKERT Matthias MUHR Verena HIRSCH Thomas CORSTJENS Paul TANKE Hans SOUKKA Tero GORRIS Hans

Rok publikování 2016
Druh Článek v odborném periodiku
Časopis / Zdroj Analytical Chemistry
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www http://pubs.acs.org/doi/full/10.1021/acs.analchem.5b04147
Doi http://dx.doi.org/10.1021/acs.analchem.5b04147
Obor Analytická chemie, separace
Klíčová slova CONVERTING PHOSPHOR REPORTERS; LANTHANIDE-DOPED NANOCRYSTALS; UP-CONVERSION NANOPARTICLES; CIRCULATING ANODIC ANTIGEN; LATERAL FLOW ASSAY; IMAGING IN-VIVO; FLUORESCENT; CELLS; NANOPHOSPHORS; LUMINESCENCE
Popis An upconversion laser scanner has been optimized to exploit the advantages of photon-upconverting nanoparticles (UCNPs) for background-free imaging on a macroscopic scale. A collimated 980 nm laser beam afforded high local excitation densities to account for the nonlinear luminescence response of UCNPs. As few as 2000 nano particles were detectable, and the linear dynamic range covered more than 5 orders of magnitude, which is essentially impossible by using conventional fluorescent dyes. UCNPs covered by a dye-doped silica shell were separated by agarose gel electrophoresis and scanned by a conventional fluorescence scanner as well as the upconversion scanner. Both optical labels could be detected independently. Finally, upconversion images of lateral flow test strips were recorded to facilitate the sensitive and quantitative detection of disease markers. A marker for the parasitic worm Schistosoma was used in this study.
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