Structural and functional characterization of lectin AFL1 from Aspergillus fumigatus

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Authors	KOMÁREK Jan KOSTLÁNOVÁ Nikola VARROT Annabelle IMBERTY Anne WIMMEROVÁ Michaela
Year of publication	2009
Type	Article in Proceedings
Conference	XIII. Setkání biochemiků a molekulárních biologů
MU Faculty or unit	Faculty of Science
Citation
Field	Biochemistry
Keywords	lectin Aspergillus fumigatus
Description	This study deals with structural and functional characterization of 34.6 kDa lectin AFL1 from ascomycete Aspergillus fumigatus. AFL1 was expressed in a recombinant form and purified by FPLC on mannose-agarose column. Binding properties of AFL1 were determined by SPR and ITC. Several promising crystals for X-ray diffraction have been obtained using hanging drop vapour diffusion technique combined with streak seeding technique.
Related projects:	Proteins in metabolism and interaction of organisms with the environment Lectins from human pathogens - structure, function, engineering