Stanovení alloxanu v mouce pomocí kapilární elektroforézy ve spojení s tandemovou hmotnostní spektrometrií

Title in English Determinal of Alloxan in Flour by Capillary Electrophoresis with Tandem Mass Spectrometry
Authors

HORSKÁ Jana OBŠÍVAČOVÁ Alena VÍTKOVÁ Kateřina JURČÍKOVÁ Jana PROCHÁZKA Václav PETR Jan

Year of publication 2020
Type Article in Periodical
Magazine / Source Chemické Listy
MU Faculty or unit

Faculty of Education

Citation
Web http://www.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/3639/3576
Keywords alloxan; diabetes; mass spectrometry; capillary electrophoresis
Description We have developed a fast and efficient method for the determination of alloxan in flour samples by capillary electrophoresis with tandem mass spectrometry without derivatization step. Mass spectra of alloxan were studied in detail providing characteristic signals at m/z 159, 141 and 115. The separation lasted 8 min under the optimum conditions using 50 mM ammonium acetate pH 9.5 as the background electrolyte and uric acid as the internal standard. Five flour samples including bleached flour from the USA were analyzed and alloxan was not detected in any of these samples.

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