Fitting competitive adsorption isotherms to the distribution data in normal phase systems with binary mobile phases
| Autoři | |
|---|---|
| Rok publikování | 1999 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Journal of Chromatography A |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TG8-3VN3JY4-1&_user=10&_coverDate=01%2F29%2F1999&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1397071277&_rerunOrigin=google&_acct=C000050221&_version=1&_urlVersion=0&_u |
| Obor | Analytická chemie, separace |
| Klíčová slova | Benzophenone; coumarine; adsorption isotherms; mobile phase compositions |
| Popis | To investigate the effects of the solvents on the overloaded separations in normal-phase systems, the distribution isotherms of benzophenone and of coumarin as model compounds were determined between a silica gel column and binary solvents containing various concentrations of 2-propanol in n-hexane and in dichloromethane and of dichloromethane in n-heptane. The distribution data in the individual solvent systems can be formally described by the Langmuir adsorption isotherm, but the quadratic isotherm generally improves the fit to the experimental data. The validity of the equations proposed to describe the distribution data in normal-phase systems was verified by comparison of the numerically calculated and the experimental band profiles. |